Pelagia Research Library
Advances in Applied comprehension Research, 2011, 2 (2): 265-271
ISSN: 0976-8610 CODEN (USA): AASRFC
Aqueous two phase pedigree of Lipase from sift Bran
Mukesh Kumar Sharma Institute of Biotechnology and Allied Sciences, Piprali Road, Sikar (Rajasthan) ______________________________________________________________________________ ABSTRACT Due to heterogeneous application of lipase in medical and industrial field, aqueous two-phase stock for the downstream processing of lipase has been exploited. The influence of system parameters such as phase forming salts, molecular weight of the phase forming polymer, system pH, inter-group communication line length, and phase volume ratio on the partition off behavior of lipase was evaluated. The aqueous two-phase system consisting of PEG 4000 and Magnesium sulfate and it has shown better results with the purification of 1.648 fold. As PEG 4000 with Magnesium sulphate has shown better results so further work can be carried out using PEG 4000 as phase forming polymer. Keywords: crystallineliquid extraction - Lipase - Aqueous two phase extraction - Partition coefficient, Lipase, Rice Bran.
______________________________________________________________________________ INTRODUCTION Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are ubiquitous enzymes of large physiological significance and industrial potential. Lipase catalyze the hydrolysis of triacylglcerols to glycerol and withdraw fatty acids at oil-water interface. They have extensive application in food, pharmaceutical, paper, cosmetic, detergent, and leather industries. Recent developments in biotechnology have loose up new avenues towards the production of many enzymes/proteins of important in research, pharmaceutical and industrial applications. There has been an increased interest in the development of efficient down streaming processing methods for separation, assimilation and purification of...If you want to get a full essay, rove it on our website: Ordercustompaper.com
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